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Block class
1 semester- 1 credit/ Quarter
The course consists first of a brief review and then further explorationof the study of heredity. Includes a basic review of genetics and molecular biology (functions of DNA) and then builds on these concepts in a laboratory setting. Lab activites include: bacterial transformation, recombinant transformation, DNA fringerprinting, and individual genetic marker identification, with the use of polymerase chain reaction cuminating in actually sequencing a region of each students DNA. Other topics covered will include genetically modified plants and animals, the human genome project, bionformatics, and the ethical issues surounding the area of biology.
Week 1&2
Introduce concept of biotechnology and molecular biology. Investigate basic questions and history of discoveries. Review basic genetics,
Lab activities:
Human DNA Isolation
Week 3 & 4 Review and expand DNA/RNA operations of replication, transcription, and translation
Bacterial transformation – ampicilin resistance, Red Colony, pGLO
Protein isolation
Week 5 & 6 Review bacterial Genetic engineering
Lab activities
Recombinant Lab
Week 7, 8, 9 Review and expand DNA finger printing,
Lab Activities
Restriction analysis
PCR - VNTR, SNP,
Week 10,11,12 Introduce DNA sequencing
Lab Activities
mtDNA sequencing
Week 13 & 14 Review and expand Human Genome project & Bioinformatics
Lab activities: dnai - Gene boy activity
Week 15, 16,17 Genetically modified organisms
Week 18 Bioethics